Staining:Article Title: IFNα-induced BST2 + tumor-associated macrophages facilitate immunosuppression and tumor growth in pancreatic cancer by ERK-CXCL7 signaling.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bovine Serum Albumin Sigma-Aldrich Cat#: A1933 3, 30-diaminobenzidine ZSGB-BIO Cat#: ZLI-9019 Hematoxylin Solarbio Life Sciences Cat#: H8070 40,6-diamidino-2-phenylindole Staining Solution Beyotime Cat#: C1005 RIPA lysis buffer Beyotime Cat#: P0013C Protease and phosphatase inhibitor cocktail Beyotime Cat#: P1045 PMSF Beyotime Cat#: ST506 Super-sensitive ECL chemiluminescent substrate Biosharp Cat#: BL520A Crystal violet staining solution Beyotime Cat#: C0121 DNase I Sigma-Aldrich Cat#: DN25 Collagenase, Type IV Gibco Cat#: 17104-019 Percoll GE Healthcare Cat#: 17-0891-09 103 Red Blood Cell Lysing Buffer BD Biosciences Cat#: 555899 Fixation Buffer BioLegend Cat#: 420801 Cell Staining Buffer BioLegend Cat#: 420201 Intracellular Staining Perm Wash Buffer (10X) BioLegend Cat#: 421002 HitransG P (40X) Genechem N/A jetPRIME Polyplus Cat#: 101000046 Critical commercial assays CD8+ T cell Isolation Kit Miltenyi Biotec Cat#: 130-104-075 Lymphocyte separation medium TBD Cat#: LTS1077 Opal PolarisTM 7-Color IHC Kit Akoya Biosciences Cat#: NEL861001KT BCA Protein Assay Reagent Kit Beyotime Cat#: P0011 Tissue Mitochondria Isolation Kit Beyotime Cat#: C3606 RNA-Quick Purification Kit Yishan Biotechnology Cat#: ES-RN001 HiScript II Reverse Transcriptase kit Vazyme Cat#: R223 AceQ qPCR SYBR Green Master Mix Vazyme Cat#: Q111-02/03 pHrodo Deep Red BioParticles Conjugate reagent Thermo Fisher Scientific Cat#: P36600 Mouse Cytokine Array GS4000 RayBiotech Cat#: GSM-CAA-4000 Mouse L-Selectin ELISA KIT Solarbio Life Sciences Cat#: SEKM-0106 Mouse CCL5 (RANTES) ELISA KIT Solarbio Life Sciences Cat#: SEKM-0043 Mouse MMP-2 ELISA KIT Solarbio Life Sciences Cat#: SEKM-0166 Mouse Colony Stimulating Factor 1, Macrophage (MCSF) ELISA Kit Reddot Biotech Cat#: RD-MCSF-Mu Mouse IL-6 ELISA KIT Solarbio Life Sciences Cat#: SEKM-0007 Human CXCL7/NAP-2 ELISA KIT Solarbio Life Sciences Cat#: SEKH-0503 Mouse bTG/PBP/CXCL7/NAP2 ELISA Kit Elabscience Biotechnology Cat#: E-EL-M1269 Human IFN alpha ELISA Kit Proteintech Cat#: KE00207 Mouse IFN alpha ELISA Kit Thermo Fisher Scientific Cat#: BMS6027 Reactive Oxygen Species Assay Kit Beyotime Cat#: S0033M Mitochondrial membrane potential assay kit with JC-1 Beyotime Cat#: C2006 QIAamp DNA Mini Kits QIAGEN Cat#: 51104 Annexin V-APC/7-AAD Apoptosis Kit Multisciences Cat#: AP105-100 DNA Damage Assay Kit by g-H2AX Immunofluorescence Beyotime Cat#: C2035S (Continued on next page) Cell Reports 43, 114088, April 23, 2024 23
Article Title: IFNα-induced BST2 + tumor-associated macrophages facilitate immunosuppression and tumor growth in pancreatic cancer by ERK-CXCL7 signaling.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data Single-cell RNA-seq Chen et al., 202331 GSE212966 Transcriptomic RNA-seq Lin et al., 201938 GSE119794 All source data associated with paper This paper https://doi.org/10.17632/2wc8f2t4np.1 Experimental models: Cell lines THP-1 American Type Culture Collection TIB-202 Experimental models: Organisms/strains Mouse: C57BL/6 Hangzhou Ziyuan C57BL/6 Mouse: C57BL/6 BST2flox/WT Cyagen Biosciences S-CKO-14564 Mouse: C57BL/6 Lyz2-Cre Cyagen Biosciences C001003 Mouse: C57BL/6 BST2flox/flox; Lyz2-Cre This paper N/A Oligonucleotides Primers See Table S1 N/A siBST2 See Table S1 N/A siCXCL7 See Table S1 N/A Recombinant DNA Mouse BST-2 CRISPR Plasmids Santa Cruz Biotechnology sc-427447 Software and algorithms FlowJo software TreeStar RRID: SCR_008520 ImageJ software National Institutes of Health RRID: SCR_003070 GraphPad Prism https://www.graphpad.com/ RRID: SCR_002798 R studio https://www.rstudio.com/ N/A
Lysis:Article Title: IFNα-induced BST2 + tumor-associated macrophages facilitate immunosuppression and tumor growth in pancreatic cancer by ERK-CXCL7 signaling.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bovine Serum Albumin Sigma-Aldrich Cat#: A1933 3, 30-diaminobenzidine ZSGB-BIO Cat#: ZLI-9019 Hematoxylin Solarbio Life Sciences Cat#: H8070 40,6-diamidino-2-phenylindole Staining Solution Beyotime Cat#: C1005 RIPA lysis buffer Beyotime Cat#: P0013C Protease and phosphatase inhibitor cocktail Beyotime Cat#: P1045 PMSF Beyotime Cat#: ST506 Super-sensitive ECL chemiluminescent substrate Biosharp Cat#: BL520A Crystal violet staining solution Beyotime Cat#: C0121 DNase I Sigma-Aldrich Cat#: DN25 Collagenase, Type IV Gibco Cat#: 17104-019 Percoll GE Healthcare Cat#: 17-0891-09 103 Red Blood Cell Lysing Buffer BD Biosciences Cat#: 555899 Fixation Buffer BioLegend Cat#: 420801 Cell Staining Buffer BioLegend Cat#: 420201 Intracellular Staining Perm Wash Buffer (10X) BioLegend Cat#: 421002 HitransG P (40X) Genechem N/A jetPRIME Polyplus Cat#: 101000046 Critical commercial assays CD8+ T cell Isolation Kit Miltenyi Biotec Cat#: 130-104-075 Lymphocyte separation medium TBD Cat#: LTS1077 Opal PolarisTM 7-Color IHC Kit Akoya Biosciences Cat#: NEL861001KT BCA Protein Assay Reagent Kit Beyotime Cat#: P0011 Tissue Mitochondria Isolation Kit Beyotime Cat#: C3606 RNA-Quick Purification Kit Yishan Biotechnology Cat#: ES-RN001 HiScript II Reverse Transcriptase kit Vazyme Cat#: R223 AceQ qPCR SYBR Green Master Mix Vazyme Cat#: Q111-02/03 pHrodo Deep Red BioParticles Conjugate reagent Thermo Fisher Scientific Cat#: P36600 Mouse Cytokine Array GS4000 RayBiotech Cat#: GSM-CAA-4000 Mouse L-Selectin ELISA KIT Solarbio Life Sciences Cat#: SEKM-0106 Mouse CCL5 (RANTES) ELISA KIT Solarbio Life Sciences Cat#: SEKM-0043 Mouse MMP-2 ELISA KIT Solarbio Life Sciences Cat#: SEKM-0166 Mouse Colony Stimulating Factor 1, Macrophage (MCSF) ELISA Kit Reddot Biotech Cat#: RD-MCSF-Mu Mouse IL-6 ELISA KIT Solarbio Life Sciences Cat#: SEKM-0007 Human CXCL7/NAP-2 ELISA KIT Solarbio Life Sciences Cat#: SEKH-0503 Mouse bTG/PBP/CXCL7/NAP2 ELISA Kit Elabscience Biotechnology Cat#: E-EL-M1269 Human IFN alpha ELISA Kit Proteintech Cat#: KE00207 Mouse IFN alpha ELISA Kit Thermo Fisher Scientific Cat#: BMS6027 Reactive Oxygen Species Assay Kit Beyotime Cat#: S0033M Mitochondrial membrane potential assay kit with JC-1 Beyotime Cat#: C2006 QIAamp DNA Mini Kits QIAGEN Cat#: 51104 Annexin V-APC/7-AAD Apoptosis Kit Multisciences Cat#: AP105-100 DNA Damage Assay Kit by g-H2AX Immunofluorescence Beyotime Cat#: C2035S (Continued on next page) Cell Reports 43, 114088, April 23, 2024 23
Article Title: IFNα-induced BST2 + tumor-associated macrophages facilitate immunosuppression and tumor growth in pancreatic cancer by ERK-CXCL7 signaling.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data Single-cell RNA-seq Chen et al., 202331 GSE212966 Transcriptomic RNA-seq Lin et al., 201938 GSE119794 All source data associated with paper This paper https://doi.org/10.17632/2wc8f2t4np.1 Experimental models: Cell lines THP-1 American Type Culture Collection TIB-202 Experimental models: Organisms/strains Mouse: C57BL/6 Hangzhou Ziyuan C57BL/6 Mouse: C57BL/6 BST2flox/WT Cyagen Biosciences S-CKO-14564 Mouse: C57BL/6 Lyz2-Cre Cyagen Biosciences C001003 Mouse: C57BL/6 BST2flox/flox; Lyz2-Cre This paper N/A Oligonucleotides Primers See Table S1 N/A siBST2 See Table S1 N/A siCXCL7 See Table S1 N/A Recombinant DNA Mouse BST-2 CRISPR Plasmids Santa Cruz Biotechnology sc-427447 Software and algorithms FlowJo software TreeStar RRID: SCR_008520 ImageJ software National Institutes of Health RRID: SCR_003070 GraphPad Prism https://www.graphpad.com/ RRID: SCR_002798 R studio https://www.rstudio.com/ N/A
Cell Isolation:Article Title: IFNα-induced BST2 + tumor-associated macrophages facilitate immunosuppression and tumor growth in pancreatic cancer by ERK-CXCL7 signaling.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bovine Serum Albumin Sigma-Aldrich Cat#: A1933 3, 30-diaminobenzidine ZSGB-BIO Cat#: ZLI-9019 Hematoxylin Solarbio Life Sciences Cat#: H8070 40,6-diamidino-2-phenylindole Staining Solution Beyotime Cat#: C1005 RIPA lysis buffer Beyotime Cat#: P0013C Protease and phosphatase inhibitor cocktail Beyotime Cat#: P1045 PMSF Beyotime Cat#: ST506 Super-sensitive ECL chemiluminescent substrate Biosharp Cat#: BL520A Crystal violet staining solution Beyotime Cat#: C0121 DNase I Sigma-Aldrich Cat#: DN25 Collagenase, Type IV Gibco Cat#: 17104-019 Percoll GE Healthcare Cat#: 17-0891-09 103 Red Blood Cell Lysing Buffer BD Biosciences Cat#: 555899 Fixation Buffer BioLegend Cat#: 420801 Cell Staining Buffer BioLegend Cat#: 420201 Intracellular Staining Perm Wash Buffer (10X) BioLegend Cat#: 421002 HitransG P (40X) Genechem N/A jetPRIME Polyplus Cat#: 101000046 Critical commercial assays CD8+ T cell Isolation Kit Miltenyi Biotec Cat#: 130-104-075 Lymphocyte separation medium TBD Cat#: LTS1077 Opal PolarisTM 7-Color IHC Kit Akoya Biosciences Cat#: NEL861001KT BCA Protein Assay Reagent Kit Beyotime Cat#: P0011 Tissue Mitochondria Isolation Kit Beyotime Cat#: C3606 RNA-Quick Purification Kit Yishan Biotechnology Cat#: ES-RN001 HiScript II Reverse Transcriptase kit Vazyme Cat#: R223 AceQ qPCR SYBR Green Master Mix Vazyme Cat#: Q111-02/03 pHrodo Deep Red BioParticles Conjugate reagent Thermo Fisher Scientific Cat#: P36600 Mouse Cytokine Array GS4000 RayBiotech Cat#: GSM-CAA-4000 Mouse L-Selectin ELISA KIT Solarbio Life Sciences Cat#: SEKM-0106 Mouse CCL5 (RANTES) ELISA KIT Solarbio Life Sciences Cat#: SEKM-0043 Mouse MMP-2 ELISA KIT Solarbio Life Sciences Cat#: SEKM-0166 Mouse Colony Stimulating Factor 1, Macrophage (MCSF) ELISA Kit Reddot Biotech Cat#: RD-MCSF-Mu Mouse IL-6 ELISA KIT Solarbio Life Sciences Cat#: SEKM-0007 Human CXCL7/NAP-2 ELISA KIT Solarbio Life Sciences Cat#: SEKH-0503 Mouse bTG/PBP/CXCL7/NAP2 ELISA Kit Elabscience Biotechnology Cat#: E-EL-M1269 Human IFN alpha ELISA Kit Proteintech Cat#: KE00207 Mouse IFN alpha ELISA Kit Thermo Fisher Scientific Cat#: BMS6027 Reactive Oxygen Species Assay Kit Beyotime Cat#: S0033M Mitochondrial membrane potential assay kit with JC-1 Beyotime Cat#: C2006 QIAamp DNA Mini Kits QIAGEN Cat#: 51104 Annexin V-APC/7-AAD Apoptosis Kit Multisciences Cat#: AP105-100 DNA Damage Assay Kit by g-H2AX Immunofluorescence Beyotime Cat#: C2035S (Continued on next page) Cell Reports 43, 114088, April 23, 2024 23
Article Title: IFNα-induced BST2 + tumor-associated macrophages facilitate immunosuppression and tumor growth in pancreatic cancer by ERK-CXCL7 signaling.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data Single-cell RNA-seq Chen et al., 202331 GSE212966 Transcriptomic RNA-seq Lin et al., 201938 GSE119794 All source data associated with paper This paper https://doi.org/10.17632/2wc8f2t4np.1 Experimental models: Cell lines THP-1 American Type Culture Collection TIB-202 Experimental models: Organisms/strains Mouse: C57BL/6 Hangzhou Ziyuan C57BL/6 Mouse: C57BL/6 BST2flox/WT Cyagen Biosciences S-CKO-14564 Mouse: C57BL/6 Lyz2-Cre Cyagen Biosciences C001003 Mouse: C57BL/6 BST2flox/flox; Lyz2-Cre This paper N/A Oligonucleotides Primers See Table S1 N/A siBST2 See Table S1 N/A siCXCL7 See Table S1 N/A Recombinant DNA Mouse BST-2 CRISPR Plasmids Santa Cruz Biotechnology sc-427447 Software and algorithms FlowJo software TreeStar RRID: SCR_008520 ImageJ software National Institutes of Health RRID: SCR_003070 GraphPad Prism https://www.graphpad.com/ RRID: SCR_002798 R studio https://www.rstudio.com/ N/A
Immunohistochemistry:Article Title: IFNα-induced BST2 + tumor-associated macrophages facilitate immunosuppression and tumor growth in pancreatic cancer by ERK-CXCL7 signaling.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bovine Serum Albumin Sigma-Aldrich Cat#: A1933 3, 30-diaminobenzidine ZSGB-BIO Cat#: ZLI-9019 Hematoxylin Solarbio Life Sciences Cat#: H8070 40,6-diamidino-2-phenylindole Staining Solution Beyotime Cat#: C1005 RIPA lysis buffer Beyotime Cat#: P0013C Protease and phosphatase inhibitor cocktail Beyotime Cat#: P1045 PMSF Beyotime Cat#: ST506 Super-sensitive ECL chemiluminescent substrate Biosharp Cat#: BL520A Crystal violet staining solution Beyotime Cat#: C0121 DNase I Sigma-Aldrich Cat#: DN25 Collagenase, Type IV Gibco Cat#: 17104-019 Percoll GE Healthcare Cat#: 17-0891-09 103 Red Blood Cell Lysing Buffer BD Biosciences Cat#: 555899 Fixation Buffer BioLegend Cat#: 420801 Cell Staining Buffer BioLegend Cat#: 420201 Intracellular Staining Perm Wash Buffer (10X) BioLegend Cat#: 421002 HitransG P (40X) Genechem N/A jetPRIME Polyplus Cat#: 101000046 Critical commercial assays CD8+ T cell Isolation Kit Miltenyi Biotec Cat#: 130-104-075 Lymphocyte separation medium TBD Cat#: LTS1077 Opal PolarisTM 7-Color IHC Kit Akoya Biosciences Cat#: NEL861001KT BCA Protein Assay Reagent Kit Beyotime Cat#: P0011 Tissue Mitochondria Isolation Kit Beyotime Cat#: C3606 RNA-Quick Purification Kit Yishan Biotechnology Cat#: ES-RN001 HiScript II Reverse Transcriptase kit Vazyme Cat#: R223 AceQ qPCR SYBR Green Master Mix Vazyme Cat#: Q111-02/03 pHrodo Deep Red BioParticles Conjugate reagent Thermo Fisher Scientific Cat#: P36600 Mouse Cytokine Array GS4000 RayBiotech Cat#: GSM-CAA-4000 Mouse L-Selectin ELISA KIT Solarbio Life Sciences Cat#: SEKM-0106 Mouse CCL5 (RANTES) ELISA KIT Solarbio Life Sciences Cat#: SEKM-0043 Mouse MMP-2 ELISA KIT Solarbio Life Sciences Cat#: SEKM-0166 Mouse Colony Stimulating Factor 1, Macrophage (MCSF) ELISA Kit Reddot Biotech Cat#: RD-MCSF-Mu Mouse IL-6 ELISA KIT Solarbio Life Sciences Cat#: SEKM-0007 Human CXCL7/NAP-2 ELISA KIT Solarbio Life Sciences Cat#: SEKH-0503 Mouse bTG/PBP/CXCL7/NAP2 ELISA Kit Elabscience Biotechnology Cat#: E-EL-M1269 Human IFN alpha ELISA Kit Proteintech Cat#: KE00207 Mouse IFN alpha ELISA Kit Thermo Fisher Scientific Cat#: BMS6027 Reactive Oxygen Species Assay Kit Beyotime Cat#: S0033M Mitochondrial membrane potential assay kit with JC-1 Beyotime Cat#: C2006 QIAamp DNA Mini Kits QIAGEN Cat#: 51104 Annexin V-APC/7-AAD Apoptosis Kit Multisciences Cat#: AP105-100 DNA Damage Assay Kit by g-H2AX Immunofluorescence Beyotime Cat#: C2035S (Continued on next page) Cell Reports 43, 114088, April 23, 2024 23
Article Title: IFNα-induced BST2 + tumor-associated macrophages facilitate immunosuppression and tumor growth in pancreatic cancer by ERK-CXCL7 signaling.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data Single-cell RNA-seq Chen et al., 202331 GSE212966 Transcriptomic RNA-seq Lin et al., 201938 GSE119794 All source data associated with paper This paper https://doi.org/10.17632/2wc8f2t4np.1 Experimental models: Cell lines THP-1 American Type Culture Collection TIB-202 Experimental models: Organisms/strains Mouse: C57BL/6 Hangzhou Ziyuan C57BL/6 Mouse: C57BL/6 BST2flox/WT Cyagen Biosciences S-CKO-14564 Mouse: C57BL/6 Lyz2-Cre Cyagen Biosciences C001003 Mouse: C57BL/6 BST2flox/flox; Lyz2-Cre This paper N/A Oligonucleotides Primers See Table S1 N/A siBST2 See Table S1 N/A siCXCL7 See Table S1 N/A Recombinant DNA Mouse BST-2 CRISPR Plasmids Santa Cruz Biotechnology sc-427447 Software and algorithms FlowJo software TreeStar RRID: SCR_008520 ImageJ software National Institutes of Health RRID: SCR_003070 GraphPad Prism https://www.graphpad.com/ RRID: SCR_002798 R studio https://www.rstudio.com/ N/A
Bicinchoninic Acid Protein Assay:Article Title: IFNα-induced BST2 + tumor-associated macrophages facilitate immunosuppression and tumor growth in pancreatic cancer by ERK-CXCL7 signaling.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bovine Serum Albumin Sigma-Aldrich Cat#: A1933 3, 30-diaminobenzidine ZSGB-BIO Cat#: ZLI-9019 Hematoxylin Solarbio Life Sciences Cat#: H8070 40,6-diamidino-2-phenylindole Staining Solution Beyotime Cat#: C1005 RIPA lysis buffer Beyotime Cat#: P0013C Protease and phosphatase inhibitor cocktail Beyotime Cat#: P1045 PMSF Beyotime Cat#: ST506 Super-sensitive ECL chemiluminescent substrate Biosharp Cat#: BL520A Crystal violet staining solution Beyotime Cat#: C0121 DNase I Sigma-Aldrich Cat#: DN25 Collagenase, Type IV Gibco Cat#: 17104-019 Percoll GE Healthcare Cat#: 17-0891-09 103 Red Blood Cell Lysing Buffer BD Biosciences Cat#: 555899 Fixation Buffer BioLegend Cat#: 420801 Cell Staining Buffer BioLegend Cat#: 420201 Intracellular Staining Perm Wash Buffer (10X) BioLegend Cat#: 421002 HitransG P (40X) Genechem N/A jetPRIME Polyplus Cat#: 101000046 Critical commercial assays CD8+ T cell Isolation Kit Miltenyi Biotec Cat#: 130-104-075 Lymphocyte separation medium TBD Cat#: LTS1077 Opal PolarisTM 7-Color IHC Kit Akoya Biosciences Cat#: NEL861001KT BCA Protein Assay Reagent Kit Beyotime Cat#: P0011 Tissue Mitochondria Isolation Kit Beyotime Cat#: C3606 RNA-Quick Purification Kit Yishan Biotechnology Cat#: ES-RN001 HiScript II Reverse Transcriptase kit Vazyme Cat#: R223 AceQ qPCR SYBR Green Master Mix Vazyme Cat#: Q111-02/03 pHrodo Deep Red BioParticles Conjugate reagent Thermo Fisher Scientific Cat#: P36600 Mouse Cytokine Array GS4000 RayBiotech Cat#: GSM-CAA-4000 Mouse L-Selectin ELISA KIT Solarbio Life Sciences Cat#: SEKM-0106 Mouse CCL5 (RANTES) ELISA KIT Solarbio Life Sciences Cat#: SEKM-0043 Mouse MMP-2 ELISA KIT Solarbio Life Sciences Cat#: SEKM-0166 Mouse Colony Stimulating Factor 1, Macrophage (MCSF) ELISA Kit Reddot Biotech Cat#: RD-MCSF-Mu Mouse IL-6 ELISA KIT Solarbio Life Sciences Cat#: SEKM-0007 Human CXCL7/NAP-2 ELISA KIT Solarbio Life Sciences Cat#: SEKH-0503 Mouse bTG/PBP/CXCL7/NAP2 ELISA Kit Elabscience Biotechnology Cat#: E-EL-M1269 Human IFN alpha ELISA Kit Proteintech Cat#: KE00207 Mouse IFN alpha ELISA Kit Thermo Fisher Scientific Cat#: BMS6027 Reactive Oxygen Species Assay Kit Beyotime Cat#: S0033M Mitochondrial membrane potential assay kit with JC-1 Beyotime Cat#: C2006 QIAamp DNA Mini Kits QIAGEN Cat#: 51104 Annexin V-APC/7-AAD Apoptosis Kit Multisciences Cat#: AP105-100 DNA Damage Assay Kit by g-H2AX Immunofluorescence Beyotime Cat#: C2035S (Continued on next page) Cell Reports 43, 114088, April 23, 2024 23
Article Title: IFNα-induced BST2 + tumor-associated macrophages facilitate immunosuppression and tumor growth in pancreatic cancer by ERK-CXCL7 signaling.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data Single-cell RNA-seq Chen et al., 202331 GSE212966 Transcriptomic RNA-seq Lin et al., 201938 GSE119794 All source data associated with paper This paper https://doi.org/10.17632/2wc8f2t4np.1 Experimental models: Cell lines THP-1 American Type Culture Collection TIB-202 Experimental models: Organisms/strains Mouse: C57BL/6 Hangzhou Ziyuan C57BL/6 Mouse: C57BL/6 BST2flox/WT Cyagen Biosciences S-CKO-14564 Mouse: C57BL/6 Lyz2-Cre Cyagen Biosciences C001003 Mouse: C57BL/6 BST2flox/flox; Lyz2-Cre This paper N/A Oligonucleotides Primers See Table S1 N/A siBST2 See Table S1 N/A siCXCL7 See Table S1 N/A Recombinant DNA Mouse BST-2 CRISPR Plasmids Santa Cruz Biotechnology sc-427447 Software and algorithms FlowJo software TreeStar RRID: SCR_008520 ImageJ software National Institutes of Health RRID: SCR_003070 GraphPad Prism https://www.graphpad.com/ RRID: SCR_002798 R studio https://www.rstudio.com/ N/A
Isolation:Article Title: IFNα-induced BST2 + tumor-associated macrophages facilitate immunosuppression and tumor growth in pancreatic cancer by ERK-CXCL7 signaling.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bovine Serum Albumin Sigma-Aldrich Cat#: A1933 3, 30-diaminobenzidine ZSGB-BIO Cat#: ZLI-9019 Hematoxylin Solarbio Life Sciences Cat#: H8070 40,6-diamidino-2-phenylindole Staining Solution Beyotime Cat#: C1005 RIPA lysis buffer Beyotime Cat#: P0013C Protease and phosphatase inhibitor cocktail Beyotime Cat#: P1045 PMSF Beyotime Cat#: ST506 Super-sensitive ECL chemiluminescent substrate Biosharp Cat#: BL520A Crystal violet staining solution Beyotime Cat#: C0121 DNase I Sigma-Aldrich Cat#: DN25 Collagenase, Type IV Gibco Cat#: 17104-019 Percoll GE Healthcare Cat#: 17-0891-09 103 Red Blood Cell Lysing Buffer BD Biosciences Cat#: 555899 Fixation Buffer BioLegend Cat#: 420801 Cell Staining Buffer BioLegend Cat#: 420201 Intracellular Staining Perm Wash Buffer (10X) BioLegend Cat#: 421002 HitransG P (40X) Genechem N/A jetPRIME Polyplus Cat#: 101000046 Critical commercial assays CD8+ T cell Isolation Kit Miltenyi Biotec Cat#: 130-104-075 Lymphocyte separation medium TBD Cat#: LTS1077 Opal PolarisTM 7-Color IHC Kit Akoya Biosciences Cat#: NEL861001KT BCA Protein Assay Reagent Kit Beyotime Cat#: P0011 Tissue Mitochondria Isolation Kit Beyotime Cat#: C3606 RNA-Quick Purification Kit Yishan Biotechnology Cat#: ES-RN001 HiScript II Reverse Transcriptase kit Vazyme Cat#: R223 AceQ qPCR SYBR Green Master Mix Vazyme Cat#: Q111-02/03 pHrodo Deep Red BioParticles Conjugate reagent Thermo Fisher Scientific Cat#: P36600 Mouse Cytokine Array GS4000 RayBiotech Cat#: GSM-CAA-4000 Mouse L-Selectin ELISA KIT Solarbio Life Sciences Cat#: SEKM-0106 Mouse CCL5 (RANTES) ELISA KIT Solarbio Life Sciences Cat#: SEKM-0043 Mouse MMP-2 ELISA KIT Solarbio Life Sciences Cat#: SEKM-0166 Mouse Colony Stimulating Factor 1, Macrophage (MCSF) ELISA Kit Reddot Biotech Cat#: RD-MCSF-Mu Mouse IL-6 ELISA KIT Solarbio Life Sciences Cat#: SEKM-0007 Human CXCL7/NAP-2 ELISA KIT Solarbio Life Sciences Cat#: SEKH-0503 Mouse bTG/PBP/CXCL7/NAP2 ELISA Kit Elabscience Biotechnology Cat#: E-EL-M1269 Human IFN alpha ELISA Kit Proteintech Cat#: KE00207 Mouse IFN alpha ELISA Kit Thermo Fisher Scientific Cat#: BMS6027 Reactive Oxygen Species Assay Kit Beyotime Cat#: S0033M Mitochondrial membrane potential assay kit with JC-1 Beyotime Cat#: C2006 QIAamp DNA Mini Kits QIAGEN Cat#: 51104 Annexin V-APC/7-AAD Apoptosis Kit Multisciences Cat#: AP105-100 DNA Damage Assay Kit by g-H2AX Immunofluorescence Beyotime Cat#: C2035S (Continued on next page) Cell Reports 43, 114088, April 23, 2024 23
Article Title: IFNα-induced BST2 + tumor-associated macrophages facilitate immunosuppression and tumor growth in pancreatic cancer by ERK-CXCL7 signaling.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data Single-cell RNA-seq Chen et al., 202331 GSE212966 Transcriptomic RNA-seq Lin et al., 201938 GSE119794 All source data associated with paper This paper https://doi.org/10.17632/2wc8f2t4np.1 Experimental models: Cell lines THP-1 American Type Culture Collection TIB-202 Experimental models: Organisms/strains Mouse: C57BL/6 Hangzhou Ziyuan C57BL/6 Mouse: C57BL/6 BST2flox/WT Cyagen Biosciences S-CKO-14564 Mouse: C57BL/6 Lyz2-Cre Cyagen Biosciences C001003 Mouse: C57BL/6 BST2flox/flox; Lyz2-Cre This paper N/A Oligonucleotides Primers See Table S1 N/A siBST2 See Table S1 N/A siCXCL7 See Table S1 N/A Recombinant DNA Mouse BST-2 CRISPR Plasmids Santa Cruz Biotechnology sc-427447 Software and algorithms FlowJo software TreeStar RRID: SCR_008520 ImageJ software National Institutes of Health RRID: SCR_003070 GraphPad Prism https://www.graphpad.com/ RRID: SCR_002798 R studio https://www.rstudio.com/ N/A
Purification:Article Title: IFNα-induced BST2 + tumor-associated macrophages facilitate immunosuppression and tumor growth in pancreatic cancer by ERK-CXCL7 signaling.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bovine Serum Albumin Sigma-Aldrich Cat#: A1933 3, 30-diaminobenzidine ZSGB-BIO Cat#: ZLI-9019 Hematoxylin Solarbio Life Sciences Cat#: H8070 40,6-diamidino-2-phenylindole Staining Solution Beyotime Cat#: C1005 RIPA lysis buffer Beyotime Cat#: P0013C Protease and phosphatase inhibitor cocktail Beyotime Cat#: P1045 PMSF Beyotime Cat#: ST506 Super-sensitive ECL chemiluminescent substrate Biosharp Cat#: BL520A Crystal violet staining solution Beyotime Cat#: C0121 DNase I Sigma-Aldrich Cat#: DN25 Collagenase, Type IV Gibco Cat#: 17104-019 Percoll GE Healthcare Cat#: 17-0891-09 103 Red Blood Cell Lysing Buffer BD Biosciences Cat#: 555899 Fixation Buffer BioLegend Cat#: 420801 Cell Staining Buffer BioLegend Cat#: 420201 Intracellular Staining Perm Wash Buffer (10X) BioLegend Cat#: 421002 HitransG P (40X) Genechem N/A jetPRIME Polyplus Cat#: 101000046 Critical commercial assays CD8+ T cell Isolation Kit Miltenyi Biotec Cat#: 130-104-075 Lymphocyte separation medium TBD Cat#: LTS1077 Opal PolarisTM 7-Color IHC Kit Akoya Biosciences Cat#: NEL861001KT BCA Protein Assay Reagent Kit Beyotime Cat#: P0011 Tissue Mitochondria Isolation Kit Beyotime Cat#: C3606 RNA-Quick Purification Kit Yishan Biotechnology Cat#: ES-RN001 HiScript II Reverse Transcriptase kit Vazyme Cat#: R223 AceQ qPCR SYBR Green Master Mix Vazyme Cat#: Q111-02/03 pHrodo Deep Red BioParticles Conjugate reagent Thermo Fisher Scientific Cat#: P36600 Mouse Cytokine Array GS4000 RayBiotech Cat#: GSM-CAA-4000 Mouse L-Selectin ELISA KIT Solarbio Life Sciences Cat#: SEKM-0106 Mouse CCL5 (RANTES) ELISA KIT Solarbio Life Sciences Cat#: SEKM-0043 Mouse MMP-2 ELISA KIT Solarbio Life Sciences Cat#: SEKM-0166 Mouse Colony Stimulating Factor 1, Macrophage (MCSF) ELISA Kit Reddot Biotech Cat#: RD-MCSF-Mu Mouse IL-6 ELISA KIT Solarbio Life Sciences Cat#: SEKM-0007 Human CXCL7/NAP-2 ELISA KIT Solarbio Life Sciences Cat#: SEKH-0503 Mouse bTG/PBP/CXCL7/NAP2 ELISA Kit Elabscience Biotechnology Cat#: E-EL-M1269 Human IFN alpha ELISA Kit Proteintech Cat#: KE00207 Mouse IFN alpha ELISA Kit Thermo Fisher Scientific Cat#: BMS6027 Reactive Oxygen Species Assay Kit Beyotime Cat#: S0033M Mitochondrial membrane potential assay kit with JC-1 Beyotime Cat#: C2006 QIAamp DNA Mini Kits QIAGEN Cat#: 51104 Annexin V-APC/7-AAD Apoptosis Kit Multisciences Cat#: AP105-100 DNA Damage Assay Kit by g-H2AX Immunofluorescence Beyotime Cat#: C2035S (Continued on next page) Cell Reports 43, 114088, April 23, 2024 23
Article Title: IFNα-induced BST2 + tumor-associated macrophages facilitate immunosuppression and tumor growth in pancreatic cancer by ERK-CXCL7 signaling.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data Single-cell RNA-seq Chen et al., 202331 GSE212966 Transcriptomic RNA-seq Lin et al., 201938 GSE119794 All source data associated with paper This paper https://doi.org/10.17632/2wc8f2t4np.1 Experimental models: Cell lines THP-1 American Type Culture Collection TIB-202 Experimental models: Organisms/strains Mouse: C57BL/6 Hangzhou Ziyuan C57BL/6 Mouse: C57BL/6 BST2flox/WT Cyagen Biosciences S-CKO-14564 Mouse: C57BL/6 Lyz2-Cre Cyagen Biosciences C001003 Mouse: C57BL/6 BST2flox/flox; Lyz2-Cre This paper N/A Oligonucleotides Primers See Table S1 N/A siBST2 See Table S1 N/A siCXCL7 See Table S1 N/A Recombinant DNA Mouse BST-2 CRISPR Plasmids Santa Cruz Biotechnology sc-427447 Software and algorithms FlowJo software TreeStar RRID: SCR_008520 ImageJ software National Institutes of Health RRID: SCR_003070 GraphPad Prism https://www.graphpad.com/ RRID: SCR_002798 R studio https://www.rstudio.com/ N/A
Reverse Transcription:Article Title: IFNα-induced BST2 + tumor-associated macrophages facilitate immunosuppression and tumor growth in pancreatic cancer by ERK-CXCL7 signaling.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bovine Serum Albumin Sigma-Aldrich Cat#: A1933 3, 30-diaminobenzidine ZSGB-BIO Cat#: ZLI-9019 Hematoxylin Solarbio Life Sciences Cat#: H8070 40,6-diamidino-2-phenylindole Staining Solution Beyotime Cat#: C1005 RIPA lysis buffer Beyotime Cat#: P0013C Protease and phosphatase inhibitor cocktail Beyotime Cat#: P1045 PMSF Beyotime Cat#: ST506 Super-sensitive ECL chemiluminescent substrate Biosharp Cat#: BL520A Crystal violet staining solution Beyotime Cat#: C0121 DNase I Sigma-Aldrich Cat#: DN25 Collagenase, Type IV Gibco Cat#: 17104-019 Percoll GE Healthcare Cat#: 17-0891-09 103 Red Blood Cell Lysing Buffer BD Biosciences Cat#: 555899 Fixation Buffer BioLegend Cat#: 420801 Cell Staining Buffer BioLegend Cat#: 420201 Intracellular Staining Perm Wash Buffer (10X) BioLegend Cat#: 421002 HitransG P (40X) Genechem N/A jetPRIME Polyplus Cat#: 101000046 Critical commercial assays CD8+ T cell Isolation Kit Miltenyi Biotec Cat#: 130-104-075 Lymphocyte separation medium TBD Cat#: LTS1077 Opal PolarisTM 7-Color IHC Kit Akoya Biosciences Cat#: NEL861001KT BCA Protein Assay Reagent Kit Beyotime Cat#: P0011 Tissue Mitochondria Isolation Kit Beyotime Cat#: C3606 RNA-Quick Purification Kit Yishan Biotechnology Cat#: ES-RN001 HiScript II Reverse Transcriptase kit Vazyme Cat#: R223 AceQ qPCR SYBR Green Master Mix Vazyme Cat#: Q111-02/03 pHrodo Deep Red BioParticles Conjugate reagent Thermo Fisher Scientific Cat#: P36600 Mouse Cytokine Array GS4000 RayBiotech Cat#: GSM-CAA-4000 Mouse L-Selectin ELISA KIT Solarbio Life Sciences Cat#: SEKM-0106 Mouse CCL5 (RANTES) ELISA KIT Solarbio Life Sciences Cat#: SEKM-0043 Mouse MMP-2 ELISA KIT Solarbio Life Sciences Cat#: SEKM-0166 Mouse Colony Stimulating Factor 1, Macrophage (MCSF) ELISA Kit Reddot Biotech Cat#: RD-MCSF-Mu Mouse IL-6 ELISA KIT Solarbio Life Sciences Cat#: SEKM-0007 Human CXCL7/NAP-2 ELISA KIT Solarbio Life Sciences Cat#: SEKH-0503 Mouse bTG/PBP/CXCL7/NAP2 ELISA Kit Elabscience Biotechnology Cat#: E-EL-M1269 Human IFN alpha ELISA Kit Proteintech Cat#: KE00207 Mouse IFN alpha ELISA Kit Thermo Fisher Scientific Cat#: BMS6027 Reactive Oxygen Species Assay Kit Beyotime Cat#: S0033M Mitochondrial membrane potential assay kit with JC-1 Beyotime Cat#: C2006 QIAamp DNA Mini Kits QIAGEN Cat#: 51104 Annexin V-APC/7-AAD Apoptosis Kit Multisciences Cat#: AP105-100 DNA Damage Assay Kit by g-H2AX Immunofluorescence Beyotime Cat#: C2035S (Continued on next page) Cell Reports 43, 114088, April 23, 2024 23
Article Title: IFNα-induced BST2 + tumor-associated macrophages facilitate immunosuppression and tumor growth in pancreatic cancer by ERK-CXCL7 signaling.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data Single-cell RNA-seq Chen et al., 202331 GSE212966 Transcriptomic RNA-seq Lin et al., 201938 GSE119794 All source data associated with paper This paper https://doi.org/10.17632/2wc8f2t4np.1 Experimental models: Cell lines THP-1 American Type Culture Collection TIB-202 Experimental models: Organisms/strains Mouse: C57BL/6 Hangzhou Ziyuan C57BL/6 Mouse: C57BL/6 BST2flox/WT Cyagen Biosciences S-CKO-14564 Mouse: C57BL/6 Lyz2-Cre Cyagen Biosciences C001003 Mouse: C57BL/6 BST2flox/flox; Lyz2-Cre This paper N/A Oligonucleotides Primers See Table S1 N/A siBST2 See Table S1 N/A siCXCL7 See Table S1 N/A Recombinant DNA Mouse BST-2 CRISPR Plasmids Santa Cruz Biotechnology sc-427447 Software and algorithms FlowJo software TreeStar RRID: SCR_008520 ImageJ software National Institutes of Health RRID: SCR_003070 GraphPad Prism https://www.graphpad.com/ RRID: SCR_002798 R studio https://www.rstudio.com/ N/A
Real-time Polymerase Chain Reaction:Article Title: IFNα-induced BST2 + tumor-associated macrophages facilitate immunosuppression and tumor growth in pancreatic cancer by ERK-CXCL7 signaling.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bovine Serum Albumin Sigma-Aldrich Cat#: A1933 3, 30-diaminobenzidine ZSGB-BIO Cat#: ZLI-9019 Hematoxylin Solarbio Life Sciences Cat#: H8070 40,6-diamidino-2-phenylindole Staining Solution Beyotime Cat#: C1005 RIPA lysis buffer Beyotime Cat#: P0013C Protease and phosphatase inhibitor cocktail Beyotime Cat#: P1045 PMSF Beyotime Cat#: ST506 Super-sensitive ECL chemiluminescent substrate Biosharp Cat#: BL520A Crystal violet staining solution Beyotime Cat#: C0121 DNase I Sigma-Aldrich Cat#: DN25 Collagenase, Type IV Gibco Cat#: 17104-019 Percoll GE Healthcare Cat#: 17-0891-09 103 Red Blood Cell Lysing Buffer BD Biosciences Cat#: 555899 Fixation Buffer BioLegend Cat#: 420801 Cell Staining Buffer BioLegend Cat#: 420201 Intracellular Staining Perm Wash Buffer (10X) BioLegend Cat#: 421002 HitransG P (40X) Genechem N/A jetPRIME Polyplus Cat#: 101000046 Critical commercial assays CD8+ T cell Isolation Kit Miltenyi Biotec Cat#: 130-104-075 Lymphocyte separation medium TBD Cat#: LTS1077 Opal PolarisTM 7-Color IHC Kit Akoya Biosciences Cat#: NEL861001KT BCA Protein Assay Reagent Kit Beyotime Cat#: P0011 Tissue Mitochondria Isolation Kit Beyotime Cat#: C3606 RNA-Quick Purification Kit Yishan Biotechnology Cat#: ES-RN001 HiScript II Reverse Transcriptase kit Vazyme Cat#: R223 AceQ qPCR SYBR Green Master Mix Vazyme Cat#: Q111-02/03 pHrodo Deep Red BioParticles Conjugate reagent Thermo Fisher Scientific Cat#: P36600 Mouse Cytokine Array GS4000 RayBiotech Cat#: GSM-CAA-4000 Mouse L-Selectin ELISA KIT Solarbio Life Sciences Cat#: SEKM-0106 Mouse CCL5 (RANTES) ELISA KIT Solarbio Life Sciences Cat#: SEKM-0043 Mouse MMP-2 ELISA KIT Solarbio Life Sciences Cat#: SEKM-0166 Mouse Colony Stimulating Factor 1, Macrophage (MCSF) ELISA Kit Reddot Biotech Cat#: RD-MCSF-Mu Mouse IL-6 ELISA KIT Solarbio Life Sciences Cat#: SEKM-0007 Human CXCL7/NAP-2 ELISA KIT Solarbio Life Sciences Cat#: SEKH-0503 Mouse bTG/PBP/CXCL7/NAP2 ELISA Kit Elabscience Biotechnology Cat#: E-EL-M1269 Human IFN alpha ELISA Kit Proteintech Cat#: KE00207 Mouse IFN alpha ELISA Kit Thermo Fisher Scientific Cat#: BMS6027 Reactive Oxygen Species Assay Kit Beyotime Cat#: S0033M Mitochondrial membrane potential assay kit with JC-1 Beyotime Cat#: C2006 QIAamp DNA Mini Kits QIAGEN Cat#: 51104 Annexin V-APC/7-AAD Apoptosis Kit Multisciences Cat#: AP105-100 DNA Damage Assay Kit by g-H2AX Immunofluorescence Beyotime Cat#: C2035S (Continued on next page) Cell Reports 43, 114088, April 23, 2024 23
Article Title: IFNα-induced BST2 + tumor-associated macrophages facilitate immunosuppression and tumor growth in pancreatic cancer by ERK-CXCL7 signaling.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data Single-cell RNA-seq Chen et al., 202331 GSE212966 Transcriptomic RNA-seq Lin et al., 201938 GSE119794 All source data associated with paper This paper https://doi.org/10.17632/2wc8f2t4np.1 Experimental models: Cell lines THP-1 American Type Culture Collection TIB-202 Experimental models: Organisms/strains Mouse: C57BL/6 Hangzhou Ziyuan C57BL/6 Mouse: C57BL/6 BST2flox/WT Cyagen Biosciences S-CKO-14564 Mouse: C57BL/6 Lyz2-Cre Cyagen Biosciences C001003 Mouse: C57BL/6 BST2flox/flox; Lyz2-Cre This paper N/A Oligonucleotides Primers See Table S1 N/A siBST2 See Table S1 N/A siCXCL7 See Table S1 N/A Recombinant DNA Mouse BST-2 CRISPR Plasmids Santa Cruz Biotechnology sc-427447 Software and algorithms FlowJo software TreeStar RRID: SCR_008520 ImageJ software National Institutes of Health RRID: SCR_003070 GraphPad Prism https://www.graphpad.com/ RRID: SCR_002798 R studio https://www.rstudio.com/ N/A
SYBR Green Assay:Article Title: IFNα-induced BST2 + tumor-associated macrophages facilitate immunosuppression and tumor growth in pancreatic cancer by ERK-CXCL7 signaling.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bovine Serum Albumin Sigma-Aldrich Cat#: A1933 3, 30-diaminobenzidine ZSGB-BIO Cat#: ZLI-9019 Hematoxylin Solarbio Life Sciences Cat#: H8070 40,6-diamidino-2-phenylindole Staining Solution Beyotime Cat#: C1005 RIPA lysis buffer Beyotime Cat#: P0013C Protease and phosphatase inhibitor cocktail Beyotime Cat#: P1045 PMSF Beyotime Cat#: ST506 Super-sensitive ECL chemiluminescent substrate Biosharp Cat#: BL520A Crystal violet staining solution Beyotime Cat#: C0121 DNase I Sigma-Aldrich Cat#: DN25 Collagenase, Type IV Gibco Cat#: 17104-019 Percoll GE Healthcare Cat#: 17-0891-09 103 Red Blood Cell Lysing Buffer BD Biosciences Cat#: 555899 Fixation Buffer BioLegend Cat#: 420801 Cell Staining Buffer BioLegend Cat#: 420201 Intracellular Staining Perm Wash Buffer (10X) BioLegend Cat#: 421002 HitransG P (40X) Genechem N/A jetPRIME Polyplus Cat#: 101000046 Critical commercial assays CD8+ T cell Isolation Kit Miltenyi Biotec Cat#: 130-104-075 Lymphocyte separation medium TBD Cat#: LTS1077 Opal PolarisTM 7-Color IHC Kit Akoya Biosciences Cat#: NEL861001KT BCA Protein Assay Reagent Kit Beyotime Cat#: P0011 Tissue Mitochondria Isolation Kit Beyotime Cat#: C3606 RNA-Quick Purification Kit Yishan Biotechnology Cat#: ES-RN001 HiScript II Reverse Transcriptase kit Vazyme Cat#: R223 AceQ qPCR SYBR Green Master Mix Vazyme Cat#: Q111-02/03 pHrodo Deep Red BioParticles Conjugate reagent Thermo Fisher Scientific Cat#: P36600 Mouse Cytokine Array GS4000 RayBiotech Cat#: GSM-CAA-4000 Mouse L-Selectin ELISA KIT Solarbio Life Sciences Cat#: SEKM-0106 Mouse CCL5 (RANTES) ELISA KIT Solarbio Life Sciences Cat#: SEKM-0043 Mouse MMP-2 ELISA KIT Solarbio Life Sciences Cat#: SEKM-0166 Mouse Colony Stimulating Factor 1, Macrophage (MCSF) ELISA Kit Reddot Biotech Cat#: RD-MCSF-Mu Mouse IL-6 ELISA KIT Solarbio Life Sciences Cat#: SEKM-0007 Human CXCL7/NAP-2 ELISA KIT Solarbio Life Sciences Cat#: SEKH-0503 Mouse bTG/PBP/CXCL7/NAP2 ELISA Kit Elabscience Biotechnology Cat#: E-EL-M1269 Human IFN alpha ELISA Kit Proteintech Cat#: KE00207 Mouse IFN alpha ELISA Kit Thermo Fisher Scientific Cat#: BMS6027 Reactive Oxygen Species Assay Kit Beyotime Cat#: S0033M Mitochondrial membrane potential assay kit with JC-1 Beyotime Cat#: C2006 QIAamp DNA Mini Kits QIAGEN Cat#: 51104 Annexin V-APC/7-AAD Apoptosis Kit Multisciences Cat#: AP105-100 DNA Damage Assay Kit by g-H2AX Immunofluorescence Beyotime Cat#: C2035S (Continued on next page) Cell Reports 43, 114088, April 23, 2024 23
Article Title: IFNα-induced BST2 + tumor-associated macrophages facilitate immunosuppression and tumor growth in pancreatic cancer by ERK-CXCL7 signaling.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data Single-cell RNA-seq Chen et al., 202331 GSE212966 Transcriptomic RNA-seq Lin et al., 201938 GSE119794 All source data associated with paper This paper https://doi.org/10.17632/2wc8f2t4np.1 Experimental models: Cell lines THP-1 American Type Culture Collection TIB-202 Experimental models: Organisms/strains Mouse: C57BL/6 Hangzhou Ziyuan C57BL/6 Mouse: C57BL/6 BST2flox/WT Cyagen Biosciences S-CKO-14564 Mouse: C57BL/6 Lyz2-Cre Cyagen Biosciences C001003 Mouse: C57BL/6 BST2flox/flox; Lyz2-Cre This paper N/A Oligonucleotides Primers See Table S1 N/A siBST2 See Table S1 N/A siCXCL7 See Table S1 N/A Recombinant DNA Mouse BST-2 CRISPR Plasmids Santa Cruz Biotechnology sc-427447 Software and algorithms FlowJo software TreeStar RRID: SCR_008520 ImageJ software National Institutes of Health RRID: SCR_003070 GraphPad Prism https://www.graphpad.com/ RRID: SCR_002798 R studio https://www.rstudio.com/ N/A
Enzyme-linked Immunosorbent Assay:Article Title: IFNα-induced BST2 + tumor-associated macrophages facilitate immunosuppression and tumor growth in pancreatic cancer by ERK-CXCL7 signaling.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bovine Serum Albumin Sigma-Aldrich Cat#: A1933 3, 30-diaminobenzidine ZSGB-BIO Cat#: ZLI-9019 Hematoxylin Solarbio Life Sciences Cat#: H8070 40,6-diamidino-2-phenylindole Staining Solution Beyotime Cat#: C1005 RIPA lysis buffer Beyotime Cat#: P0013C Protease and phosphatase inhibitor cocktail Beyotime Cat#: P1045 PMSF Beyotime Cat#: ST506 Super-sensitive ECL chemiluminescent substrate Biosharp Cat#: BL520A Crystal violet staining solution Beyotime Cat#: C0121 DNase I Sigma-Aldrich Cat#: DN25 Collagenase, Type IV Gibco Cat#: 17104-019 Percoll GE Healthcare Cat#: 17-0891-09 103 Red Blood Cell Lysing Buffer BD Biosciences Cat#: 555899 Fixation Buffer BioLegend Cat#: 420801 Cell Staining Buffer BioLegend Cat#: 420201 Intracellular Staining Perm Wash Buffer (10X) BioLegend Cat#: 421002 HitransG P (40X) Genechem N/A jetPRIME Polyplus Cat#: 101000046 Critical commercial assays CD8+ T cell Isolation Kit Miltenyi Biotec Cat#: 130-104-075 Lymphocyte separation medium TBD Cat#: LTS1077 Opal PolarisTM 7-Color IHC Kit Akoya Biosciences Cat#: NEL861001KT BCA Protein Assay Reagent Kit Beyotime Cat#: P0011 Tissue Mitochondria Isolation Kit Beyotime Cat#: C3606 RNA-Quick Purification Kit Yishan Biotechnology Cat#: ES-RN001 HiScript II Reverse Transcriptase kit Vazyme Cat#: R223 AceQ qPCR SYBR Green Master Mix Vazyme Cat#: Q111-02/03 pHrodo Deep Red BioParticles Conjugate reagent Thermo Fisher Scientific Cat#: P36600 Mouse Cytokine Array GS4000 RayBiotech Cat#: GSM-CAA-4000 Mouse L-Selectin ELISA KIT Solarbio Life Sciences Cat#: SEKM-0106 Mouse CCL5 (RANTES) ELISA KIT Solarbio Life Sciences Cat#: SEKM-0043 Mouse MMP-2 ELISA KIT Solarbio Life Sciences Cat#: SEKM-0166 Mouse Colony Stimulating Factor 1, Macrophage (MCSF) ELISA Kit Reddot Biotech Cat#: RD-MCSF-Mu Mouse IL-6 ELISA KIT Solarbio Life Sciences Cat#: SEKM-0007 Human CXCL7/NAP-2 ELISA KIT Solarbio Life Sciences Cat#: SEKH-0503 Mouse bTG/PBP/CXCL7/NAP2 ELISA Kit Elabscience Biotechnology Cat#: E-EL-M1269 Human IFN alpha ELISA Kit Proteintech Cat#: KE00207 Mouse IFN alpha ELISA Kit Thermo Fisher Scientific Cat#: BMS6027 Reactive Oxygen Species Assay Kit Beyotime Cat#: S0033M Mitochondrial membrane potential assay kit with JC-1 Beyotime Cat#: C2006 QIAamp DNA Mini Kits QIAGEN Cat#: 51104 Annexin V-APC/7-AAD Apoptosis Kit Multisciences Cat#: AP105-100 DNA Damage Assay Kit by g-H2AX Immunofluorescence Beyotime Cat#: C2035S (Continued on next page) Cell Reports 43, 114088, April 23, 2024 23
Article Title: IFNα-induced BST2 + tumor-associated macrophages facilitate immunosuppression and tumor growth in pancreatic cancer by ERK-CXCL7 signaling.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data Single-cell RNA-seq Chen et al., 202331 GSE212966 Transcriptomic RNA-seq Lin et al., 201938 GSE119794 All source data associated with paper This paper https://doi.org/10.17632/2wc8f2t4np.1 Experimental models: Cell lines THP-1 American Type Culture Collection TIB-202 Experimental models: Organisms/strains Mouse: C57BL/6 Hangzhou Ziyuan C57BL/6 Mouse: C57BL/6 BST2flox/WT Cyagen Biosciences S-CKO-14564 Mouse: C57BL/6 Lyz2-Cre Cyagen Biosciences C001003 Mouse: C57BL/6 BST2flox/flox; Lyz2-Cre This paper N/A Oligonucleotides Primers See Table S1 N/A siBST2 See Table S1 N/A siCXCL7 See Table S1 N/A Recombinant DNA Mouse BST-2 CRISPR Plasmids Santa Cruz Biotechnology sc-427447 Software and algorithms FlowJo software TreeStar RRID: SCR_008520 ImageJ software National Institutes of Health RRID: SCR_003070 GraphPad Prism https://www.graphpad.com/ RRID: SCR_002798 R studio https://www.rstudio.com/ N/A
Membrane:Article Title: IFNα-induced BST2 + tumor-associated macrophages facilitate immunosuppression and tumor growth in pancreatic cancer by ERK-CXCL7 signaling.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bovine Serum Albumin Sigma-Aldrich Cat#: A1933 3, 30-diaminobenzidine ZSGB-BIO Cat#: ZLI-9019 Hematoxylin Solarbio Life Sciences Cat#: H8070 40,6-diamidino-2-phenylindole Staining Solution Beyotime Cat#: C1005 RIPA lysis buffer Beyotime Cat#: P0013C Protease and phosphatase inhibitor cocktail Beyotime Cat#: P1045 PMSF Beyotime Cat#: ST506 Super-sensitive ECL chemiluminescent substrate Biosharp Cat#: BL520A Crystal violet staining solution Beyotime Cat#: C0121 DNase I Sigma-Aldrich Cat#: DN25 Collagenase, Type IV Gibco Cat#: 17104-019 Percoll GE Healthcare Cat#: 17-0891-09 103 Red Blood Cell Lysing Buffer BD Biosciences Cat#: 555899 Fixation Buffer BioLegend Cat#: 420801 Cell Staining Buffer BioLegend Cat#: 420201 Intracellular Staining Perm Wash Buffer (10X) BioLegend Cat#: 421002 HitransG P (40X) Genechem N/A jetPRIME Polyplus Cat#: 101000046 Critical commercial assays CD8+ T cell Isolation Kit Miltenyi Biotec Cat#: 130-104-075 Lymphocyte separation medium TBD Cat#: LTS1077 Opal PolarisTM 7-Color IHC Kit Akoya Biosciences Cat#: NEL861001KT BCA Protein Assay Reagent Kit Beyotime Cat#: P0011 Tissue Mitochondria Isolation Kit Beyotime Cat#: C3606 RNA-Quick Purification Kit Yishan Biotechnology Cat#: ES-RN001 HiScript II Reverse Transcriptase kit Vazyme Cat#: R223 AceQ qPCR SYBR Green Master Mix Vazyme Cat#: Q111-02/03 pHrodo Deep Red BioParticles Conjugate reagent Thermo Fisher Scientific Cat#: P36600 Mouse Cytokine Array GS4000 RayBiotech Cat#: GSM-CAA-4000 Mouse L-Selectin ELISA KIT Solarbio Life Sciences Cat#: SEKM-0106 Mouse CCL5 (RANTES) ELISA KIT Solarbio Life Sciences Cat#: SEKM-0043 Mouse MMP-2 ELISA KIT Solarbio Life Sciences Cat#: SEKM-0166 Mouse Colony Stimulating Factor 1, Macrophage (MCSF) ELISA Kit Reddot Biotech Cat#: RD-MCSF-Mu Mouse IL-6 ELISA KIT Solarbio Life Sciences Cat#: SEKM-0007 Human CXCL7/NAP-2 ELISA KIT Solarbio Life Sciences Cat#: SEKH-0503 Mouse bTG/PBP/CXCL7/NAP2 ELISA Kit Elabscience Biotechnology Cat#: E-EL-M1269 Human IFN alpha ELISA Kit Proteintech Cat#: KE00207 Mouse IFN alpha ELISA Kit Thermo Fisher Scientific Cat#: BMS6027 Reactive Oxygen Species Assay Kit Beyotime Cat#: S0033M Mitochondrial membrane potential assay kit with JC-1 Beyotime Cat#: C2006 QIAamp DNA Mini Kits QIAGEN Cat#: 51104 Annexin V-APC/7-AAD Apoptosis Kit Multisciences Cat#: AP105-100 DNA Damage Assay Kit by g-H2AX Immunofluorescence Beyotime Cat#: C2035S (Continued on next page) Cell Reports 43, 114088, April 23, 2024 23
Article Title: IFNα-induced BST2 + tumor-associated macrophages facilitate immunosuppression and tumor growth in pancreatic cancer by ERK-CXCL7 signaling.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data Single-cell RNA-seq Chen et al., 202331 GSE212966 Transcriptomic RNA-seq Lin et al., 201938 GSE119794 All source data associated with paper This paper https://doi.org/10.17632/2wc8f2t4np.1 Experimental models: Cell lines THP-1 American Type Culture Collection TIB-202 Experimental models: Organisms/strains Mouse: C57BL/6 Hangzhou Ziyuan C57BL/6 Mouse: C57BL/6 BST2flox/WT Cyagen Biosciences S-CKO-14564 Mouse: C57BL/6 Lyz2-Cre Cyagen Biosciences C001003 Mouse: C57BL/6 BST2flox/flox; Lyz2-Cre This paper N/A Oligonucleotides Primers See Table S1 N/A siBST2 See Table S1 N/A siCXCL7 See Table S1 N/A Recombinant DNA Mouse BST-2 CRISPR Plasmids Santa Cruz Biotechnology sc-427447 Software and algorithms FlowJo software TreeStar RRID: SCR_008520 ImageJ software National Institutes of Health RRID: SCR_003070 GraphPad Prism https://www.graphpad.com/ RRID: SCR_002798 R studio https://www.rstudio.com/ N/A
Immunofluorescence:Article Title: IFNα-induced BST2 + tumor-associated macrophages facilitate immunosuppression and tumor growth in pancreatic cancer by ERK-CXCL7 signaling.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bovine Serum Albumin Sigma-Aldrich Cat#: A1933 3, 30-diaminobenzidine ZSGB-BIO Cat#: ZLI-9019 Hematoxylin Solarbio Life Sciences Cat#: H8070 40,6-diamidino-2-phenylindole Staining Solution Beyotime Cat#: C1005 RIPA lysis buffer Beyotime Cat#: P0013C Protease and phosphatase inhibitor cocktail Beyotime Cat#: P1045 PMSF Beyotime Cat#: ST506 Super-sensitive ECL chemiluminescent substrate Biosharp Cat#: BL520A Crystal violet staining solution Beyotime Cat#: C0121 DNase I Sigma-Aldrich Cat#: DN25 Collagenase, Type IV Gibco Cat#: 17104-019 Percoll GE Healthcare Cat#: 17-0891-09 103 Red Blood Cell Lysing Buffer BD Biosciences Cat#: 555899 Fixation Buffer BioLegend Cat#: 420801 Cell Staining Buffer BioLegend Cat#: 420201 Intracellular Staining Perm Wash Buffer (10X) BioLegend Cat#: 421002 HitransG P (40X) Genechem N/A jetPRIME Polyplus Cat#: 101000046 Critical commercial assays CD8+ T cell Isolation Kit Miltenyi Biotec Cat#: 130-104-075 Lymphocyte separation medium TBD Cat#: LTS1077 Opal PolarisTM 7-Color IHC Kit Akoya Biosciences Cat#: NEL861001KT BCA Protein Assay Reagent Kit Beyotime Cat#: P0011 Tissue Mitochondria Isolation Kit Beyotime Cat#: C3606 RNA-Quick Purification Kit Yishan Biotechnology Cat#: ES-RN001 HiScript II Reverse Transcriptase kit Vazyme Cat#: R223 AceQ qPCR SYBR Green Master Mix Vazyme Cat#: Q111-02/03 pHrodo Deep Red BioParticles Conjugate reagent Thermo Fisher Scientific Cat#: P36600 Mouse Cytokine Array GS4000 RayBiotech Cat#: GSM-CAA-4000 Mouse L-Selectin ELISA KIT Solarbio Life Sciences Cat#: SEKM-0106 Mouse CCL5 (RANTES) ELISA KIT Solarbio Life Sciences Cat#: SEKM-0043 Mouse MMP-2 ELISA KIT Solarbio Life Sciences Cat#: SEKM-0166 Mouse Colony Stimulating Factor 1, Macrophage (MCSF) ELISA Kit Reddot Biotech Cat#: RD-MCSF-Mu Mouse IL-6 ELISA KIT Solarbio Life Sciences Cat#: SEKM-0007 Human CXCL7/NAP-2 ELISA KIT Solarbio Life Sciences Cat#: SEKH-0503 Mouse bTG/PBP/CXCL7/NAP2 ELISA Kit Elabscience Biotechnology Cat#: E-EL-M1269 Human IFN alpha ELISA Kit Proteintech Cat#: KE00207 Mouse IFN alpha ELISA Kit Thermo Fisher Scientific Cat#: BMS6027 Reactive Oxygen Species Assay Kit Beyotime Cat#: S0033M Mitochondrial membrane potential assay kit with JC-1 Beyotime Cat#: C2006 QIAamp DNA Mini Kits QIAGEN Cat#: 51104 Annexin V-APC/7-AAD Apoptosis Kit Multisciences Cat#: AP105-100 DNA Damage Assay Kit by g-H2AX Immunofluorescence Beyotime Cat#: C2035S (Continued on next page) Cell Reports 43, 114088, April 23, 2024 23
Article Title: IFNα-induced BST2 + tumor-associated macrophages facilitate immunosuppression and tumor growth in pancreatic cancer by ERK-CXCL7 signaling.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Deposited data Single-cell RNA-seq Chen et al., 202331 GSE212966 Transcriptomic RNA-seq Lin et al., 201938 GSE119794 All source data associated with paper This paper https://doi.org/10.17632/2wc8f2t4np.1 Experimental models: Cell lines THP-1 American Type Culture Collection TIB-202 Experimental models: Organisms/strains Mouse: C57BL/6 Hangzhou Ziyuan C57BL/6 Mouse: C57BL/6 BST2flox/WT Cyagen Biosciences S-CKO-14564 Mouse: C57BL/6 Lyz2-Cre Cyagen Biosciences C001003 Mouse: C57BL/6 BST2flox/flox; Lyz2-Cre This paper N/A Oligonucleotides Primers See Table S1 N/A siBST2 See Table S1 N/A siCXCL7 See Table S1 N/A Recombinant DNA Mouse BST-2 CRISPR Plasmids Santa Cruz Biotechnology sc-427447 Software and algorithms FlowJo software TreeStar RRID: SCR_008520 ImageJ software National Institutes of Health RRID: SCR_003070 GraphPad Prism https://www.graphpad.com/ RRID: SCR_002798 R studio https://www.rstudio.com/ N/A
|
Beyotime is a verified supplier 
Beyotime manufactures this product 